UDC 579.62: 579.253.4

POTENTIAL FOR SIMULTANEOUS GENOTYPING OF ESCHERICHIA COLI AND SALMONELLA FIELD ISOLATES AIMED AT ELUCIDATION OF ROUTES OF PATHOGEN TRANSMISSION AT POULTRY FARM SETTING

Terletskiy Valery Pavlovich1, Tyshchenko Valentina Ivanovna2, Novikova Oksana Borisovna3
1Russian Research Institute of Farm Animal Genetics and Breeding, D.Sc., Professor, senior researcher of the Laboratory of Molecular Cytogenetics
2Russian Research Institute of Farm Animal Genetics and Breeding, Cand.Sc. in biology, senior researcher of the Laboratory of Molecular Cytogenetics
3All Russia Research Veterinary Institute of Poultry Sciences, Cand.Sc. in veterinary medicine, senior researcher of the Department of Microbiology

Abstract
The genotyping technique which was used earlier for discrimination of bacterial isolates, was applied for Escherichia coli and Salmonella enterica. Search for optimal restriction endonucleases allowed for selecting pair of enzymes suitable simultaneously for these pathogen species. Data obtained will help plan veterinary measures aimed at prevention of infectious diseases in chicken.

Keywords: Escherichia coli, genotyping, prevention of infectious diseases, restriction endonucleases

Category: Agriculture

Article reference:
Terletskiy V.P., Tyshchenko V.I., Novikova O.B. Potential for simultaneous genotyping of Escherichia coli and Salmonella field isolates aimed at elucidation of routes of pathogen transmission at poultry farm setting // Agriculture, forestry and water management. 2015. № 6 [Electronic journal]. URL: https://agro.snauka.ru/en/2015/06/2422

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